Trimethylolamine methane hydrochloride CAS:1185-53-1
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Category：Biochemical Materials Own Brand：MT /MOQ:100KG /From China/ B2B only.
Introduction
Tris(hydroxymethyl)aminomethane Hydrochloride (Tris-HCl)
CAS No.: 1185-53-1
Molecular Formula: C₄H₁₁NO₃·HCl
Molecular Weight: 157.60 g/mol
1. Basic Properties
| Parameter | Specification |
|---|---|
| Appearance | White crystalline powder |
| Purity (Titration) | 99.0–101.0% |
| Solubility (1M aq.) | Colorless, clear solution |
| pH (1% aq.) | 4.2–5.0 |
| Heavy Metals | ≤5 ppm |
| UV Absorbance (1M aq.) | ≤0.06 (260 nm), ≤0.05 (280 nm) |
2. Key Applications
- Molecular Biology:
- Buffer component for DNA/RNA electrophoresis (e.g., TAE, TBE buffers)
- Protein purification and crystallization
- Organic Synthesis:
- Intermediate for pharmaceutical compounds
- Diagnostics:
- Buffer agent in IVD reagents
3. Quality Control
| Test | Acceptance Criteria |
|---|---|
| Identity (IR) | Matches reference spectrum |
| Water Content (KF) | ≤1.0% |
| Endotoxin | <0.1 EU/mg (for molecular biology grade) |
4. Packaging & Storage
| Form | Specification |
|---|---|
| Packaging | 25 kg/cardboard drum |
| Storage | – Dry, ≤25°C, ≤60% RH |
| Shelf Life | 5 years (unopened) |
5. Safety & Handling
| Hazard Code | Precaution |
|---|---|
| H315 | Causes skin irritation |
| H319 | Causes eye irritation |
| Handling | Use gloves/eye protection |
Synonyms:
Tris-HCl | Tromethamine hydrochloride
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