Dithiothreitol CAS No.:3483-12-3
Biochemical Materials 2025-03-18
DITHIOTHREITOL (DTT)
CAS No.: 3483-12-3
Molecular Formula: C₄H₁₀O₂S₂
Molecular Weight: 154.25 g/mol
1. BASIC PROPERTIES
| Parameter | Specification |
|---|---|
| Appearance | White crystalline powder |
| Purity | ≥99.0% |
| Melting Point | 41-44°C (lit.) |
| Boiling Point | 125°C |
| Density (20°C) | 1.04 g/mL |
| Flash Point | >230°F (>110°C) |
2. APPLICATIONS
- Biochemistry:
- Reduction of protein disulfide bonds (SDS-PAGE sample prep)
- Cleavage of polyacrylamide gel cross-linkers (bis(acryloyl)cystamine)
- Neuroscience:
- Prevention of peptide oxidation (cholecystokinin extraction)
- Analytical Chemistry:
- Disulfide bond analysis in protein sequencing
3. PACKAGING & STORAGE
| Form | Specification |
|---|---|
| Packaging | 25 kg/cardboard drum |
| Storage | – Dry, inert atmosphere |
| Special Notes | – Hygroscopic |
| – Argon blanket recommended for long-term storage |
4. SAFETY & HANDLING
- GHS Classification:
- H315+H319: Causes skin/eye irritation
- H335: May cause respiratory irritation
- Precautions:
- Handle under nitrogen/vacuum for sensitive applications
- Use desiccated containers
5. REGULATORY COMPLIANCE
- SDS: Available upon request
- Transport: Non-hazardous (standard conditions)
