Trimethylolamine methane hydrochloride CAS:1185-53-1
Biochemical Materials 2025-03-18
Tris(hydroxymethyl)aminomethane Hydrochloride (Tris-HCl)
CAS No.: 1185-53-1
Molecular Formula: C₄H₁₁NO₃·HCl
Molecular Weight: 157.60 g/mol
1. Basic Properties
| Parameter |
Specification |
| Appearance |
White crystalline powder |
| Purity (Titration) |
99.0–101.0% |
| Solubility (1M aq.) |
Colorless, clear solution |
| pH (1% aq.) |
4.2–5.0 |
| Heavy Metals |
≤5 ppm |
| UV Absorbance (1M aq.) |
≤0.06 (260 nm), ≤0.05 (280 nm) |
2. Key Applications
- Molecular Biology:
- Buffer component for DNA/RNA electrophoresis (e.g., TAE, TBE buffers)
- Protein purification and crystallization
- Organic Synthesis:
- Intermediate for pharmaceutical compounds
- Diagnostics:
- Buffer agent in IVD reagents
3. Quality Control
| Test |
Acceptance Criteria |
| Identity (IR) |
Matches reference spectrum |
| Water Content (KF) |
≤1.0% |
| Endotoxin |
<0.1 EU/mg (for molecular biology grade) |
4. Packaging & Storage
| Form |
Specification |
| Packaging |
25 kg/cardboard drum |
| Storage |
– Dry, ≤25°C, ≤60% RH |
| Shelf Life |
5 years (unopened) |
5. Safety & Handling
| Hazard Code |
Precaution |
| H315 |
Causes skin irritation |
| H319 |
Causes eye irritation |
| Handling |
Use gloves/eye protection |
Synonyms:
Tris-HCl | Tromethamine hydrochloride
